Growth and Nutrition of Microbes
HEMOCYTOMETRY The technique of counting of the blood cells is known as hemocytometry . This involves manual counting of the cell with the help of a microscope after diluting blood in respective diluting fluids . The cells most often counted by this technique are red cells white cells platelets and eosinophils . The differentiate the […]
Osmotic Fragility Test Of Erythrocyte INTRODUCTION Red blood cell membrane restricts the solutes and allows only water to pass through them(osmosis). if the cells are placed in hypertonic solution sodium chloride (solution concentrated than normal saline e.g. 2%, (W/V), they shrink due to exosmosis. The red cells absorb water when kept in hypertonic solution (solution […]
Preparation of Lupus Erythematosus (LE) Cell INTRODUCTION Antinuclear antibodies occur in the serum in a wide range of autoimmune disorders, including systematic lupus erythematosus (SLE). A characteristic of SLE is the presence of 7S IgG antibodies to double standard DNA (ds – DNA). LE (lupus erythematosus) cells are a type of abnormal immune cell that […]
PCV ( packed cell volume ) is the amount of packed red blood cells following centrifugation expressed as percentage of the total blood volume . Packed Cell Volume (PCV) or Hematocrit (HCT) test performe by various following Methods. Hematocrit (HCT) Test By Macro – hematocrit Clinical significance : – Fall of hematocrit value are observed […]
Cerebrospinal fluid (CSF) Analysis What is CSF ? The Cerebrospinal fluid (CSF) is formed by selective dialysis of plasma by the choroid plexus of the ventricles of the brain . Through the foramina in tha fourth ventricle it then passes into subarachnoid cisterns at the base of the brain and travels over the surface of […]
Antigen and Antibody Reaction Antigen An antigen is a substance or molecules when introduced into the body triggers the production of an antibody by the immune system. Antibody kills or neutralizes the antigen that is recognised as a foreign and potentially harmful invader. The antigen can be molecules such as pollen or cells such as […]
Definition of Colorimeter The body fluid such as blood (seram and Plasma) CSF and urine etc. contain several organic and Inorganic substances for example, blood contains organic substance such as Glucose, urea, uric acid, creatinine, Protein, etc. It also contains inorganic cations, such as sodium, potassium, calcium etc. and anion such as inorganic phosphorus chloride, […]
What is Parasite? and their Types What is Parasite? All infectious agents are parasites that are dependent on any living Organism. For nourishment, growth and multiplication. The study of parasite that cause disease in man is called as medical parasitology. Clinical parasitology deals with those pathogenic Organism which are bigger in size than bacteria and […]